D4567
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 | Immunofluorescence Staining of DNMT1 Nuclear localization COS7 cells were stained with Rabbit Anti-DNMT1 (Cat. No. D4567) at a 1:200 dilution, followed by Goat Anti-Rabbit-FITC conjugate (A). For nuclear localization, the cells were further stained with DAPI (B). |
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D4567
Sigma
Anti-DNMT1 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| Synonym: | Anti-DNA methyltransferase |
| MDL number: | MFCD06201933 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 1581-1595 of rat DNMT1, conjugated to KLH via an N-terminal added lysine residue. The sequence is conserved in human and mouse. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~180 kDa |
| species reactivity | human, mouse, rat |
| application(s) | immunoblotting: 1:1,000-1:2,000 using nuclear extracts of human HEK 293T cells |
| indirect immunofluorescence: 1:100-1:200 using mouse COS-7 cells | |
| microarray: suitable | |
| Gene Information | human ... DNMT1(1786) |
| mouse ... Dnmt1(13433) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
